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TET azide, 6-isomer

貨號 數量 價格 交貨時間
18230 1 mg $110 現貨
28230 5 mg $165 現貨
48230 25 mg $285 現貨
58230 50 mg $495 現貨
68230 100 mg $880 現貨
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TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate with absorption maximum at 519 nm and emission maximum at 535 nm. TET is spectrally similar to R6G, JOE, and VIC, widely used for labeling PCR probes.

Oligonucleotides labeled with TET are often used in real-time PCR; the preparation of such oligonucleotides can be performed using click chemistry. This derivative is an azide, a pure 6-isomer, for conjugating TET to other molecules by copper-catalyzed and copper-free click reactions.

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JOE azide, 5-isomer

JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistry

VIC azide, 6-isomer

VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.

General properties

Appearance: orange powder
Molecular weight: 596.21
CAS number: 1450752-90-5
Molecular formula: C24H14Cl4N4O6
Solubility: in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09
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