活化酯

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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貨號 數量 價格 交貨時間
2934-1mg 1 mg –   現貨
2934-25mg 25 mg $160 現貨
2934-50mg 50 mg $270 現貨
2934-100mg 100 mg $520 現貨
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AF 343(香豆素)-X-NHS 酯 (AF 343 (Coumarin)-X-NHS ester)

AF 343(香豆素)是一種藍光發射染料,可用作螢光素 (FAM) 的 FRET 供體。該結構的特點是香豆素 343 螢光團和官能團之間有一個連接體。它提供了更好的溶解性以及螢光團和官能團之間的空間分離。
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貨號 數量 價格 交貨時間
12620 1 mg $110 現貨
22620 5 mg $210 現貨
42620 25 mg $410 現貨
52620 50 mg $695 現貨
62620 100 mg $1190 現貨
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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貨號 數量 價格 交貨時間
10820 1 mg $110 現貨
20820 5 mg $165 現貨
40820 25 mg $380 現貨
50820 50 mg $695 現貨
60820 100 mg $1190 現貨
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AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯,一種明亮、光穩定、親水性的綠色通道熒光染料。
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貨號 數量 價格 交貨時間
11820 1 mg $125 現貨
21820 5 mg $325 現貨
31820 10 mg $480 現貨
41820 25 mg $950 現貨
51820 50 mg $1625 現貨
61820 100 mg $2090 現貨
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AF 488 TFP 酯 (AF 488 TFP ester)

綠色螢光染料 AF 488 的四氟苯酯,用於標記蛋白質、多肽和修飾寡核苷酸中的氨基。
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貨號 數量 價格 交貨時間
118T0 1 mg $165 現貨
218T0 5 mg $510 現貨
418T0 25 mg $1650 現貨
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AF 532 NHS 酯 (AF 532 NHS ester)

AF 532 染料的 NHS 酯,通過與氨基反應標記蛋白質、多肽和修飾寡核苷酸。
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貨號 數量 價格 交貨時間
1C820 1 mg $140 現貨
2C820 5 mg $450 現貨
4C820 25 mg $1460 現貨
5C820 50 mg $1890 現貨
6C820 100 mg $2640 現貨
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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貨號 數量 價格 交貨時間
1K820 1 mg $140 現貨
2K820 5 mg $450 現貨
4K820 25 mg $1460 現貨
5K820 50 mg $1890 現貨
6K820 100 mg $2640 現貨
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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貨號 數量 價格 交貨時間
14820 1 mg $125 現貨
24820 5 mg $325 現貨
44820 25 mg $720 現貨
54820 50 mg $1325 現貨
64820 100 mg $2090 現貨
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AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的紅色螢光 NHS-酯,用於標記蛋白質、肽、抗體和任何含有 -NH2 基團的分子。純5-異構體
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貨號 數量 價格 交貨時間
2825-1mg 1 mg $125 現貨
2825-5mg 5 mg $425 現貨
2825-25mg 25 mg $1240 現貨
2825-50mg 50 mg $1890 現貨
2825-100mg 100 mg $2640 現貨