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TET phosphoramidite, 6-isomer

TET（四氯螢光素） Oligonucleotide synthesis Phosphoramidites

貨號	數量	價格	交貨時間
C8160	100 mg	–	現貨
D8160	250 mg	$197	現貨
F8160	1 g	$575	現貨
G8160	5 g	$2265	現貨
H8160	10 g	請查詢	現貨

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TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.

TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).

TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).

5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM^® 310 Genetic Analyzer.

Recommendations for using the reagent:

Condensation: 3 min.

Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C).

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BDP® R6G maleimide

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VIC azide, 6-isomer

VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.

JOE 亞磷酰胺，6-異構體 (JOE phosphoramidite, 6-isomer)

JOE 染料亞磷酰胺用於合成標記寡核苷酸。 JOE 吸收和發射最大值介於 FAM 和 TAMRA/ROX 染料的最大值之間。

General properties

Appearance:	white solid foam
Molecular weight:	981.72
CAS number:	877049-90-6
Molecular formula:	C₄₆H₅₄N₃Cl₄O₁₀P
IUPAC name:	2',4,7,7'-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)
Solubility:	Good solubility in acetonitrile and DCM
Quality control:	NMR ¹H and ³¹P, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	519
ε, L⋅mol⁻¹⋅cm⁻¹:	100000
Emission maximum, nm:	535
Fluorescence quantum yield:	0.47
CF₂₆₀:	0.17
CF₂₈₀:	0.09

Oligo synthesis details

Diluent:

anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.