SIMA phosphoramidite, 6-isomer (hydroxyprolinol)
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 13360 | 100 mg | – | 現貨 | |
| 23360 | 250 mg | $340 | 現貨 | |
| 43360 | 1 g |
$990
|
現貨 | |
| 63360 | 5 g |
$3900
|
現貨 | |
| 83360 | 10 g | 請查詢 | 21 天 |
SIMA phosphoramidite (hydroxyprolinol), 6-isomer contains xanthene dye dichloro-diphenyl-fluorescein (SIMA) with spectral characteristics similar to those of HEX but it is considered to be more resistant to deblocking under alkaline conditions, so deprotection can be run with aqueous ammonium hydroxide at higher temperatures or with AMA (1:1 mixture, concentrated aqueous ammonium hydroxide / 40% aqueous methylamine) at room temperature for 2 h or 65 °C for 10 min.
This modifying reagent with the core of hydroxyprolinol, also has a dimethoxythrityl protection group for cleaning reversed phase HPLC (RP-HPLC), C18 column.
Recommendations for using the reagent:
Coupling: 3 min.
Deprotection: Standard conditions with 25% ammonium hydroxide; deprotection time depends on the composition of nucleic acids and their protective groups. AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine) can be used for 2 hours at room temperature or 10 min at 65 °C.
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THPTA配體
THPTA 是 Cu(I) 催化點擊化學的水溶性配體。該配體使銅穩定在 Cu(I) 氧化態。由於水溶性高，與該配體的反應不需要有機共溶劑。Chemical phosphorylation reagent
Reagent for the synthesis of 5’-phosphorylated oligonucleotides.花青3胺 (Cyanine3 amine)
Cyanine3 是一種帶有游離氨基的螢光團（氨基染料）。它可以與 NHS 酯、羧基（活化後）和環氧化物共軛。General properties
| Appearance: | white powder |
| Molecular weight: | 1480,52 |
| Molecular formula: | C84H89Cl2N4O14P |
| IUPAC name: | 6-((6-(2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)pyrrolidin-1-yl)-6-oxohexyl)carbamoyl)-4,7-dichloro-3-oxo-2',7'-diphenyl-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate) |
| Solubility: | good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 531 |
| ε, L⋅mol−1⋅cm−1: | 92300 |
| Emission maximum, nm: | 555 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.57 |
| CF280: | 0.18 |
Oligo synthesis details
| Diluent: | Anhydrous Acetonitrile |
| Coupling conditions: | 3 minute coupling time recommended |
| Deprotection conditions: | identical to protected nucleobases |
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