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FAM phosphoramidite, 5-isomer
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
2639-500mg | 500 mg | – | 現貨 | |
2639-1g | 1 g | $190 $147 | 現貨 | |
2639-5g | 5 g | $740 | 現貨 | |
2639-10g | 10 g | $1290 $970 | 現貨 | |
2639-50g | 50 g | 請查詢 | 21 天 | |
2639-100g | 100 g | 請查詢 | 21 天 |
Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
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Chemical phosphorylation reagent
Reagent for the synthesis of 5’-phosphorylated oligonucleotides.TAMRA phosphoramidite, 5-isomer
TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
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DusQ 2 phosphoramidite
DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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General properties
Appearance: | off white solid |
Molecular weight: | 843.94 |
CAS number: | 147566-42-5 |
Molecular formula: | C46H58N3O10P |
Solubility: | Good solubility in acetonitrile and DCM |
Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 492 |
ε, L⋅mol−1⋅cm−1: | 72000 |
Emission maximum, nm: | 517 |
Fluorescence quantum yield: | 0.93 |
CF260: | 0.22 |
CF280: | 0.17 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | coupling time 10 min |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine |
產品引用
- Zharkov, T. D.; Markov, O. V.; Zhukov, S. A.; Khodyreva, S. N.; Kupryushkin, M. S. Influence of Combinations of Lipophilic and Phosphate Backbone Modifications on Cellular Uptake of Modified Oligonucleotides. Molecules, 2024, 29(2), 452. doi: 10.3390/molecules29020452
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
- Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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