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FAM phosphoramidite, 5-isomer

FAM (Fluorescein) Oligonucleotide synthesis Phosphoramidites
貨號 數量 價格 交貨時間
2639-500mg 500 mg –   現貨
2639-1g 1 g $190 現貨
2639-5g 5 g $740 現貨
2639-10g 10 g $1290 現貨
2639-50g 50 g 請查詢 21 天
2639-100g 100 g 請查詢 21 天
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Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

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General properties

Appearance: off white solid
Molecular weight: 843.94
CAS number: 147566-42-5
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 72000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

產品引用

  1. Zharkov, T. D.; Markov, O. V.; Zhukov, S. A.; Khodyreva, S. N.; Kupryushkin, M. S. Influence of Combinations of Lipophilic and Phosphate Backbone Modifications on Cellular Uptake of Modified Oligonucleotides. Molecules, 2024, 29(2), 452. doi: 10.3390/molecules29020452
  2. Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
  3. Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
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