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SIMA-dT phosphoramidite, 6-isomer

貨號 數量 價格 交貨時間
2950-100mg 100 mg $180 現貨
2950-250mg 250 mg $390 現貨
2950-1g 1 g $1120 現貨
2950-5g 5 g $4520 21 天
2950-10g 10 g $8650 現貨
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SIMA-dT phosphoramidite is used to introduce SIMA in the sequence during oligonucleotide synthesis, usually as a substitute for the native dT linkage.

SIMA is known to be much more stable than HEX in basic media thus deprotection in harsh conditions using ammonium hydroxide (up to 6-8 hours at 55 °C) is possible as well as AMA at room temperature or at 65 °C.

Recommendations for using the reagent:

Coupling: 6 minutes coupling time recommended.

Deprotection: standard method recommended, can be deprotected with AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine).

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DusQ 1 amidite, 5'-terminal

Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ 1 quencher.
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Cyanine 5.5疊氮化物 (Cyanine5.5 azide)

用於點擊化學的遠紅/近紅外熒光 Cyanine5.5 染料衍生物。

SIMA 亞磷酰胺,6-異構體

一種亞磷酰胺,用於合成 SIMA 標記的寡核苷酸。SIMA 是一更穩定的HEX類似物。
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General properties

Appearance: white powder
Molecular weight: 1646.67
Molecular formula: C91H95Cl2N6O17P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 531
ε, L⋅mol−1⋅cm−1: 92300
Emission maximum, nm: 555
Fluorescence quantum yield: 0.63
CF260: 0.57
CF280: 0.18

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: identical to protected nucleobases
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