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DusQ® 1 phosphoramidite

Oligonucleotide synthesis DusQ® 1 Phosphoramidites
貨號 數量 價格 交貨時間
1470-100mg 100 mg $290 現貨
1470-500mg 500 mg $990 現貨
1470-1g 1 g $1690 現貨
1470-10g 10 g 請查詢 現貨
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DusQ® 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.

DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

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DusQ® 2 dT 亞磷酰胺 (DusQ® 2 dT phosphoramidite)

用於寡核苷酸 DusQ® 2 dT 標記的亞磷酰胺。
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DusQ 1 amidite, 5'-terminal

Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ 1 quencher.

FAM NHS 酯，6-異構體 (FAM NHS ester, 6-isomer)

活化的螢光素 N-羥基琥珀醯亞胺酯 (FAM)，純 6-異構體。

General properties

Appearance: black powder
Molecular weight: 995.11
CAS number: 374591-94-3
Molecular formula: C55H63N8O8P
IUPAC name: Phosphoramidous acid, bis(1-​methylethyl)​-​, 2-​[[2-​[bis(4-​methoxyphenyl)​phenylmethoxy]​ethyl]​[4-​[[2-​methoxy-​5-​methyl-​4-​[(4-​methyl-​2-​nitrophenyl)​azo]​phenyl]​azo]​phenyl]​amino]​ethyl 2-​cyanoethyl ester (9CI)
Solubility: good in acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 27300

Oligo synthesis details

Diluent: 50% DCM in acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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