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DusQ® 1 phosphoramidite

Oligonucleotide synthesis DusQ® 1 Phosphoramidites

貨號	數量	價格	交貨時間
1470-100mg	100 mg	$290	現貨
1470-500mg	500 mg	$990	現貨
1470-1g	1 g	$1690	現貨
1470-10g	10 g	請查詢	現貨

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DusQ^® 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5′-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.

DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

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DusQ® 1 amidite, 5'-terminal

Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ® 1 quencher.

DusQ® 1 CPG 1000

Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3'-terminal DusQ® 1 quencher. Compatible with standard deblocking conditions.

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FAM NHS 酯，6-異構體 (FAM NHS ester, 6-isomer)

活化的螢光素 N-羥基琥珀醯亞胺酯 (FAM)，純 6-異構體。

General properties

Appearance:	black powder
Molecular weight:	995.11
CAS number:	374591-94-3
Molecular formula:	C₅₅H₆₃N₈O₈P
IUPAC name:	Phosphoramidous acid, bis(1-methylethyl)-, 2-[[2-[bis(4-methoxyphenyl)phenylmethoxy]ethyl][4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)azo]phenyl]azo]phenyl]amino]ethyl 2-cyanoethyl ester (9CI)
Solubility:	good in acetonitrile
Quality control:	NMR ¹H, ³¹P, HPLC-MS (95%)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	522
ε, L⋅mol⁻¹⋅cm⁻¹:	27300

Oligo synthesis details

Diluent:	50% DCM in acetonitrile
Coupling conditions:	6 min coupling time; 3 min oxidation time
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.