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Cyanine5.5 phosphoramidite

貨號 數量 價格 交貨時間
2680-100mg 100 mg –   現貨
2680-250mg 250 mg $290 現貨
2680-1g 1 g $950 現貨
2680-10g 10 g 請查詢 5 天
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Cyanine5.5 is a fluorophore with the emission in far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.

This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.

The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.

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sulfo-Cyanine3 alkyne

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Cyanine5亞磷酰胺 (Cyanine5 phosphoramidite)

Cyanine5 亞磷酰胺衍生物,用於寡核苷酸合成
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Copper(II)-TВTA catalytic buffer, 1.5x

Ready-to-use catalytic buffer containing сopper(II) and TBTA ligand. It is suitable for click chemistry modification of nucleic acids and small molecules.

General properties

Appearance: dark colored solid
Molecular weight: 916.61
Molecular formula: C55H71N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (85%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 694
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
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