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JOE phosphoramidite, 5-isomer

JOE Oligonucleotide synthesis Phosphoramidites

貨號	數量	價格	交貨時間
C1160	100 mg	–	現貨
D1160	250 mg	$165 $66	現貨
F1160	1 g	$575 $229	現貨
G1160	5 g	$2230 $892	現貨
H1160	10 g	$3300 $1320	現貨

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JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.

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Cyanine3 NHS 酯最小染料 (Cyanine3 NHS ester minimal dye)

Cyanine3 最小染料用於蛋白質標記。

ROX NHS 酯，6-異構體

ROX（羅丹明X，R101）NHS-酯的純 6-異構體，用於胺標記。

FAM 亞磷酰胺，6-異構體 (FAM phosphoramidite, 6-isomer)

單一構體 6-FAM 亞磷酰胺，用於寡核苷酸的修飾。

General properties

Appearance:	colorless solid
Molecular weight:	972.88
Molecular formula:	C₄₈H₆₀N₃Cl₂O₁₂P
Solubility:	good in DCM, acetonitrile
Quality control:	NMR ¹H, ³¹P, HPLC-MS (95%), functional testing
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	533
ε, L⋅mol⁻¹⋅cm⁻¹:	75000
Emission maximum, nm:	554
Fluorescence quantum yield:	0.61
CF₂₆₀:	0.36
CF₂₈₀:	0.28

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.