此頁面上的某些內容不提供英文版本，將默認以當前可用的語言顯示。

DusQ 2 phosphoramidite

Oligonucleotide synthesis DusQ 2 Phosphoramidites
貨號 數量 價格 交貨時間
1473-100mg 100 mg $290 現貨
1473-500mg 500 mg $990 現貨
1473-1g 1 g $1690 現貨
1473-10g 10 g 請查詢 現貨
批量詢價
Found better price? Let us know and we will propose the way forward!

DusQ 2 phosphoramidite is suitable for the synthesis of oligonucleotides labeled with DusQ 2 quencher at the 5’- and 3’-ends, as well as in the middle of the sequence. This phosphoramidite has DMT protection, which allows purification of the synthesized 5’-labeled oligonucleotide on cartridges.

This phosphoramidite is commonly used for the synthesis of doubly labeled probes for quantitative PCR with DusQ 2 quencher at the 5’-end. DusQ 2 is a fluorescence quencher with maximum absorption in the range of 560–670 nm and is suitable for efficient quenching of fluorophores emitting in this range through FRET mechanism. It is also used in hybridization probes based on static and mixed quenching.

Since the quenching efficiency of DusQ 2 is minimally dependent on the spectral overlap between the fluorophore and the quencher, it is suitable for a wide range of fluorophores, including those with emission in the red and far-red ranges. The list of fluorophores for use with DusQ 2 includes, but is not limited to, Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

以
ChemDraw 格式下載

顧客還購買了這個產品

FAM-dT亞磷醯胺

亞磷醯胺用於在合成序列中間，用螢光素 (FAM) 標記寡核苷酸。
將此產品添加到購物車並
立即在線獲取免費快遞服務

JOE phosphoramidite, 5-isomer

JOE phosphoramidite is a xanthene dye phosphoramidite used in oligo synthesis. Its absorption and emission spectra are located between the FAM and TAMRA channels.

General properties

Appearance: dark colored solid
Molecular weight: 997.08
CAS number: 374591-98-7
Molecular formula: C54H61N8O9P
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 552
CF260: 0.31
CF280: 0.26

Oligo synthesis details

Diluent: 20% THF / acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
已添加您的物品. 查看您的 購物車 或 繼續結賬
物品數量不正確.