DusQ 2 phosphoramidite
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 1473-100mg | 100 mg | $290 | 現貨 | |
| 1473-500mg | 500 mg |
$990
|
現貨 | |
| 1473-1g | 1 g |
$1690
|
現貨 | |
| 1473-10g | 10 g | 請查詢 | 現貨 |
DusQ 2 phosphoramidite is suitable for the synthesis of oligonucleotides labeled with DusQ 2 quencher at the 5’- and 3’-ends, as well as in the middle of the sequence. This phosphoramidite has DMT protection, which allows purification of the synthesized 5’-labeled oligonucleotide on cartridges.
This phosphoramidite is commonly used for the synthesis of doubly labeled probes for quantitative PCR with DusQ 2 quencher at the 5’-end. DusQ 2 is a fluorescence quencher with maximum absorption in the range of 560–670 nm and is suitable for efficient quenching of fluorophores emitting in this range through FRET mechanism. It is also used in hybridization probes based on static and mixed quenching.
Since the quenching efficiency of DusQ 2 is minimally dependent on the spectral overlap between the fluorophore and the quencher, it is suitable for a wide range of fluorophores, including those with emission in the red and far-red ranges. The list of fluorophores for use with DusQ 2 includes, but is not limited to, Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
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Cyanine5.5亞磷醯胺 (Cyanine5.5 phosphoramidite)
Amidite 用於合成5' 末端帶有Cyanine5.5染料的寡核苷酸和 qPCR 探針。適用於多重 qPCR。
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DusQ 1 phosphoramidite
A black quencher dye phosphoramidite for the synthesis of oligonucleotide probes labeled with DusQ 1 quenchers at 5’-terminal. Useful for HEX, JOE, ROX, Cyanine3.
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General properties
| Appearance: | dark colored solid |
| Molecular weight: | 997.08 |
| CAS number: | 374591-98-7 |
| Molecular formula: | C54H61N8O9P |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Diluent: | 20% THF / acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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