此頁面上的某些內容不提供英文版本,將默認以當前可用的語言顯示。

R6G phosphoramidite, 6-isomer

貨號 數量 價格 交貨時間
C5260 100 mg –   現貨
D5260 250 mg $190 現貨
F5260 1 g $590 現貨
G5260 5 g $1950 現貨
H5260 10 g 請查詢 現貨
Found better price? Let us know and we will propose the way forward!

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

Usage:

Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

顧客還購買了這個產品

Cyanine5亞磷酰胺 (Cyanine5 phosphoramidite)

Cyanine5 亞磷酰胺衍生物,用於寡核苷酸合成

BDP® R6G azide

BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.

General properties

Appearance: yellowish powder
Molecular weight: 949.93
CAS number: 1355330-47-0
Molecular formula: C46H54F6N5O8P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
已添加您的物品. 查看您的 購物車繼續結賬
物品數量不正確.