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R6G phosphoramidite, 6-isomer

貨號 數量 價格 交貨時間
C5260 100 mg –   現貨
D5260 250 mg $190 現貨
F5260 1 g $590 現貨
G5260 5 g $1950 現貨
H5260 10 g 請查詢 現貨
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R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

Usage:

Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

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TAMRA 疊氮化物, 5-異構體 (TAMRA azide, 5-isomer)

用於點擊化學的 TAMRA 螢光染料的疊氮化物衍生物。

R6G 疊氮化物,5-異構體 (R6G azide, 5-isomer)

羅丹明 6G 是一種氧雜蒽染料,已用於 DNA 測序和 qPCR。這種疊氮化物衍生物用於通過點擊化學將染料附著到 DNA 和其他分子上。

六角疊氮化物, 6-異構體 (HEX azide, 6-isomer)

綠色-黃色發射 HEX 染料的疊氮化物,通過 [3+2]-偶極環加成與寡核苷酸偶合。

General properties

Appearance: yellowish powder
Molecular weight: 949.93
CAS number: 1355330-47-0
Molecular formula: C46H54F6N5O8P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
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