R6G phosphoramidite, 6-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| C5260 | 100 mg | – | 現貨 | |
| D5260 | 250 mg | $190 | 現貨 | |
| F5260 | 1 g |
$590
|
現貨 | |
| G5260 | 5 g |
$1950
|
現貨 | |
| H5260 | 10 g | 請查詢 | 現貨 |
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.
R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.
Usage:
Coupling: 10 minutes.
Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.
Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.
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Inosine (dI) phosphoramidite
Dimethoxytrityl deoxyinosine (Dmt-dI) phosphoramidite for the synthesis of modified DNA oligonucleotides.生物素亞磷酰胺（羥基脯氨醇）[Biotin phosphoramidite (hydroxyprolinol)]
生物素亞磷酰胺用於合成生物素化的寡核苷酸。含有二甲氧基三苯甲基基團。
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SIMA 亞磷酰胺，6-異構體
一種亞磷酰胺，用於合成 SIMA 標記的寡核苷酸。SIMA 是一更穩定的HEX類似物。
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General properties
| Appearance: | yellowish powder |
| Molecular weight: | 949.93 |
| CAS number: | 1355330-47-0 |
| Molecular formula: | C46H54F6N5O8P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 518 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
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