TAMRA CPG 500, 5-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2792-100mg | 100 mg | $95 | 現貨 | |
| 2792-500mg | 500 mg | $295 | 現貨 | |
| 2792-1g | 1 g |
$469
|
現貨 | |
| 2792-5g | 5 g | 請查詢 | 現貨 |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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FAM NHS 酯，6-異構體 (FAM NHS ester, 6-isomer)
活化的螢光素 N-羥基琥珀醯亞胺酯 (FAM)，純 6-異構體。Cyanine5.5亞磷醯胺 (Cyanine5.5 phosphoramidite)
Amidite 用於合成5' 末端帶有Cyanine5.5染料的寡核苷酸和 qPCR 探針。適用於多重 qPCR。
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生物素（羥基脯氨醇）CPG 1000
基於羥基脯氨醇的固相支持物，用於合成 3' 末端含有生物素標記的寡核苷酸。General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−70 |
產品引用
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
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