TAMRA phosphoramidite, 5-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2497-100mg | 100 mg | 請查詢 | 現貨 (3 pcs) | |
| 2497-250mg | 250 mg | 請查詢 | 現貨 (2 pcs) | |
| 2497-1g | 1 g | 請查詢 | 現貨 (2 pcs) | |
| 2497-5g | 5 g | 請查詢 | 現貨 (2 pcs) |
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
顧客還購買了這個產品
DusQ1 dT 亞磷酰胺 (DusQ 1 dT phosphoramidite)
用於寡核苷酸DusQ1 dT 標記的亞磷酰胺。
将此产品添加到购物车并立即在线获取免费快递服务证书
六角疊氮化物, 6-異構體 (HEX azide, 6-isomer)
綠色-黃色發射 HEX 染料的疊氮化物，通過 [3+2]-偶極環加成與寡核苷酸偶合。JOE 亞磷酰胺，6-異構體 (JOE phosphoramidite, 6-isomer)
JOE 染料亞磷酰胺用於合成標記寡核苷酸。 JOE 吸收和發射最大值介於 FAM 和 TAMRA/ROX 染料的最大值之間。
将此产品添加到购物车并立即在线获取免费快递服务证书
General properties
| Appearance: | purple solid |
| Mass spec M+ increment: | 589.60 |
| Molecular weight: | 727.83 |
| Molecular formula: | C40H50N5O6P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, HPLC-MS (95%), coupling test |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Diluent: | acetonitrile |
您的購物車是空的
$ 
