AF 594 streptavidin
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| A68S0 | 100 ug | – | 現貨 | |
| 168S0 | 1 mg | $185 | 現貨 |
Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.
Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.
This streptavidin is a lyophilized conjugate with AF 594, a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm).
The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.
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AF 594 NHS酯 (AF 594 NHS ester)
AF 594 染料的紅色螢光 NHS-酯，用於標記蛋白質、肽、抗體和任何含有 -NH2 基團的分子。純5-異構體dsGold 核酸凝膠染色液，10000× (dsGold Nucleic Acid Gel Staining Solution, 10,000×)
一種高靈敏度染色劑，用於電泳凝膠中的 dsDNA、ssDNA 和 RNA 染色。CM-DiI, lipophilic tracer
Orange-red fluorescent lipophilic dye for membrane labeling and cell tracking. Unlike other membrane dyes, CM-DiI persists in cells after their fixation and permeabilization.General properties
| Appearance: | purple solid |
| Solubility: | good in water |
| Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
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