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AF 594 streptavidin

AF 594 Flow cytometry Microscopy
貨號 數量 價格 交貨時間
A68S0 100 ug –   現貨
168S0 1 mg $185 現貨
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Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.

Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.

This streptavidin is a lyophilized conjugate with AF 594, a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm).

The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.

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Biotin-11-dUTP

Biotin-labeled deoxyuridine triphosphate for enzymatic incorporation into DNA and indirect detection.

AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的紅色螢光 NHS-酯，用於標記蛋白質、肽、抗體和任何含有 -NH2 基團的分子。純5-異構體
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sulfo-Cyanine3-PEG3-biotin

Conjugate of hydrophilic sulfo-Cyanine3 dye with biotin, affinity label for avidin/streptavidin.

General properties

Appearance: purple solid
Solubility: good in water
Storage conditions: Transportation: at room temperature for 1 week. Store at -20°С 9 months.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 586
ε, L⋅mol−1⋅cm−1: 105000
Emission maximum, nm: 613
Fluorescence quantum yield: 0.77
CF260: 0.28
CF280: 0.51
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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