此頁面上的某些內容不提供英文版本,將默認以當前可用的語言顯示。

sulfo-Cyanine3 streptavidin

貨號 數量 價格 交貨時間
A13S0 100 ug –   現貨
113S0 1 mg $185 現貨
Found better price? Let us know and we will propose the way forward!

Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.

Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.

This streptavidin is a lyophilized conjugate with sulfo-Cyanine3, a hydrophilic orange fluorophore with spectral characteristics similar to Cy3® (absorption max. at 548 nm, emission max. at 563 nm).

The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.

顧客還購買了這個產品

AF 488 streptavidin

AF 488 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.

Biotin-X-NHS ester

Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.

磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)

水溶性磺基-Cyanine5 琥珀酰亞胺酯,用於在水相中標記各種含胺分子。

General properties

Appearance: pink solid
Solubility: good in water
Storage conditions: Store at -20°С 9 months from date of receipt. Transportation: at room temperature for 1 week.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
已添加您的物品. 查看您的 購物車繼續結賬
物品數量不正確.