sulfo-Cyanine3 streptavidin
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| A13S0 | 100 ug | – | 現貨 | |
| 113S0 | 1 mg | $185 | 現貨 |
Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.
Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.
This streptavidin is a lyophilized conjugate with sulfo-Cyanine3, a hydrophilic orange fluorophore with spectral characteristics similar to Cy3® (absorption max. at 548 nm, emission max. at 563 nm).
The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.
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AF 594 streptavidin
AF 594 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.Biotin-PEG4-NHS ester
PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.DBCO-PEG6-NHS ester
A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).General properties
| Appearance: | pink solid |
| Solubility: | good in water |
| Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
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