6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3290-5mg | 5 mg | – | 現貨 | |
| 3290-25mg | 25 mg | $65 | 5 天 | |
| 3290-100mg | 100 mg | $150 | 現貨 |
6-Carboxy-H2DCFDA is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in living cells. This reagent is not suitable for working with fixed samples.
6-Carboxy-H2DCFDA is a non-fluorescent compound that begins to fluoresce after the cleavage of acetyl groups by cellular esterases and its oxidation by reactive oxygen species inside the cell. The resulting 6-carboxy-2’,7’-dichlorofluorescein has a bright fluorescence in the green channel (absorption maximum at 504 nm, emission maximum at 525 nm), that can be detected using various methods, such as flow cytometry, plate reading, or fluorescent microscopy.
Acetyl groups in 6-carboxy-H2DCFDA increase its lipophilicity and improve the permeability of the indicator through the cell membrane. After deacetylation by cellular esterases, the compound acquires a charge that allows it to be retained inside the cell. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.
Recommendations for using the reagent:
- Use a freshly prepared reagent solution (the working solution is not intended for long-term storage because of gradual reagent oxidation).
- Select an optimal working concentration of the reagent and incubation time required for reagent deacetylation and oxidation for the specific cell line and assay conditions. If no protocols are recommended for the specific cell line, start with a concentration from 1 to 10 µM and incubation for 30 min.
- Do not incubate the dye with the cells in the presence of serum because it contains enzymes that cleave H2DCFDA.
顧客還購買了這個產品
磺基-菁5四嗪 (sulfo-Cyanine5 tetrazine)
水溶性菁染料四嗪衍生物，用於與反式環辛烯和其他應變烯烴進行 TCO 連接反應。Pluronic® F-127
一種非離子去污劑，廣泛用於溶解疏水性分子，例如細胞滲透性AM酯類 Fluo-4、Fluorica-8 鈣指示劑和 Di-4-ANEPS、Di-8-ANEPS 電位探針，能促其加載到細胞中。dsGreen® 凝膠染色液，10000× (dsGreen® Gel Staining Solution, 10,000×)
dsGreen® 用於瓊脂糖和聚丙烯酰胺凝膠中的 dsDNA 染色。General properties
| Appearance: | white crystals |
| Molecular weight: | 531.30 |
| CAS number: | 247044-02-6 |
| Molecular formula: | C25H16Cl2O9 |
| Solubility: | DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 504 |
| ε, L⋅mol−1⋅cm−1: | 83500 |
| Emission maximum, nm: | 525 |
| Fluorescence quantum yield: | 0.79 |
| CF260: | 0.23 |
| CF280: | 0.16 |
您的購物車是空的
$ 
