6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)

顯微鏡觀察流式細胞術 ROS傳感

貨號	數量	價格	交貨時間
3290-5mg	5 mg	–	現貨
3290-25mg	25 mg	$65	現貨
3290-100mg	100 mg	$150	現貨

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6-Carboxy-H₂DCFDA is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in living cells. This reagent is not suitable for working with fixed samples.

6-Carboxy-H₂DCFDA is a non-fluorescent compound that begins to fluoresce after the cleavage of acetyl groups by cellular esterases and its oxidation by reactive oxygen species inside the cell. The resulting 6-carboxy-2’,7’-dichlorofluorescein has a bright fluorescence in the green channel (absorption maximum at 504 nm, emission maximum at 525 nm), that can be detected using various methods, such as flow cytometry, plate reading, or fluorescent microscopy.

Acetyl groups in 6-carboxy-H₂DCFDA increase its lipophilicity and improve the permeability of the indicator through the cell membrane. After deacetylation by cellular esterases, the compound acquires a charge that allows it to be retained inside the cell. This carboxylated H₂DCFDA analog has two additional negative charges that impede its leakage out of the cell.

Recommendations for using the reagent:

Use a freshly prepared reagent solution (the working solution is not intended for long-term storage because of gradual reagent oxidation).
Select an optimal working concentration of the reagent and incubation time required for reagent deacetylation and oxidation for the specific cell line and assay conditions. If no protocols are recommended for the specific cell line, start with a concentration from 1 to 10 µM and incubation for 30 min.
Do not incubate the dye with the cells in the presence of serum because it contains enzymes that cleave H₂DCFDA.

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General properties

Appearance:	white crystals
Molecular weight:	531.30
CAS number:	247044-02-6
Molecular formula:	C₂₅H₁₆Cl₂O₉
Solubility:	DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	504
ε, L⋅mol⁻¹⋅cm⁻¹:	83500
Emission maximum, nm:	525
Fluorescence quantum yield:	0.79
CF₂₆₀:	0.23
CF₂₈₀:	0.16

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.