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sulfo-Cyanine3-PEG3-biotin

貨號 數量 價格 交貨時間
3079-1mg 1 mg $120 現貨
3079-5mg 5 mg $290 現貨
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This fluorescent conjugate is useful for streptavidin-based fluorescent assays and visualization of the streptavidin/avidin-labeled biomolecules. Streptavidin, a protein with a high affinity to biotin, has four binding centers for biotin. The high stability of biotin/streptavidin complex gives the opportunity to build different kinds of assays. For example, a target molecule with affinity to the surface can be conjugated with biotin and immobilized on a solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with the biotin-dye conjugate.

The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.

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sulfo-Cyanine3 NHS酯 (sulfo-Cyanine3 NHS ester)

水溶性磺基-Cyanine3琥珀醯亞胺酯 (SE) 活化酯,用於氨基生物分子標記。

6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)

A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.

磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)

水溶性磺基-Cyanine5 琥珀酰亞胺酯,用於在水相中標記各種含胺分子。

General properties

Appearance: dark colored solid
Molecular weight: 1011.32
Molecular formula: C46H63N6KO11S3
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
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