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Proteinase K (lyophilized)

Proteinase K is a non-specific protease of the serine protease family that cleaves peptide bonds adjacent to the carboxylic group of aromatic and aliphatic amino acids. Proteinase K is commonly used to digest proteins in biological samples, remove endogenous nucleases during DNA and RNA purification, unmask antigens in immunochemistry, and prepare tissues for in situ hybridization.

Proteinase K is active under a wide range of pH (between 6.5 and 9.5), elevated temperatures, and the presence of metal chelating agents and SDS.

A typical working concentration for Proteinase K is 50–100 μg/mL.

Proteinase K is a component of LumiPure Genomic DNA Blood and Buccal Kit.

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General properties

Appearance: white powder
CAS number: 39450-01-6
Quality control: Functional test
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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