ProteOrange® Protein Gel Stain, 5000×
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 10210 | 100 uL | – | 現貨 | |
| 40210 | 1 mL | $285 | 現貨 |
ProteOrange® is a protein-binding dye for staining protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS and, therefore, can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, giving more consistent staining between different types of proteins than CBB staining and never exhibiting negative staining. It also stains glycoproteins well.
Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining and allows the detection of about 3 ng of protein per band (30 ng is the detection limit for Coumassie).
ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough to staining around 200 minigels.
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即用型試劑盒，可使用Annexin V-AF 488 結合物和碘化丙啶標記凋亡和壞死細胞。AF 488 馬來醯亞胺 (AF 488 maleimide)
AF 488 C6 馬來醯亞胺是一種用於標記蛋白質 SH 基團的硫醇反應性活性染料。General properties
| Appearance: | orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
產品引用
- Heesink, G.; van den Oetelaar, M.C.M.; Semerdzhiev, S.A.; Ottmann, C.; Brunsveld, L.; Blum, C.; Claessens, M.M.A.E. 14-3-3τ as a Modulator of Early α-Synuclein Multimerization and Amyloid Formation. ACS Chemical Neuroscience, 2024, 15(9), 1926-1936. doi: 10.1021/acschemneuro.4c00100
- Somsen, B. A.; Sijbesma, E.; Leysen, S.; Honzejkova, K.; Visser, E. J.; Cossar, P. J.; Obšil, T.; Brunsveld, L.; Ottmann, C. Molecular Basis and Dual Ligand Regulation of Tetrameric Estrogen Receptor α/14-3-3ζ Protein Complex. Journal of Biological Chemistry, 2023, 299(7), 104855. doi: 10.1016/j.jbc.2023.104855
- Vasilev, R.; Gunitseva, N.; Shebanova, R.; Korzhenkov, A.; Vlaskina, A.; Evteeva, M.; Polushkina, I.; Nikitchina, N.; Toshchakov, S.; Kamenski, P.; Patrushev, M.; Mazunin, I. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii. International Journal of Molecular Sciences, 2022, 23(16), 9289. doi: 10.3390/ijms23169289
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