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Biotin-PEG4-NHS ester

生物素化 NHS酯類交聯

貨號	數量	價格	交貨時間	購買此產品
2893-100mg	100 mg	$145	現貨
2893-1g	1 g	$590	現貨

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Biotin-PEG4-NHS ester is an activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules. The NHS ester group reacts efficiently with amino groups by the nucleophilic attack, forming an amide bond and releasing N-Hydroxysuccinimide. Biotin-labeled compounds can then be linked to avidin or streptavidin for further purification or detection. The biotin group is relatively small and doesn’t affect the biological activity of biotinylated proteins.

This reagent features a long PEG4-linker that separates the biotin residue from the target molecule, ensuring efficient binding to avidin or streptavidin. The linker also increases the compound’s water solubility, thereby facilitating bioconjugation. Antibodies labeled with Biotin-PEG4-NHS ester exhibit less aggregation when stored in solution than those labeled with reagents with only hydrocarbon spacers.

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建議協議

NHS Ester Labeling of Biomolecules Containing Primary Amino Groups

計算機

蛋白質標記計算器

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DBCO-NHS 酯

偶氮二苯並環辛炔 (DBCO、ADIBO) 試劑用於應變促進的無銅點擊化學 (SPAAC)。該試劑含有 NHS 酯官能團，用於將環辛炔連接到各種分子上。

生物素-PEG3-疊氮化物 (Biotin-PEG3-azide)

生物素疊氮化物是一種用生物素進行點擊化學標記的試劑，生物素是一種著名的鏈霉親和素親和探針。

DBCO-PEG6-NHS ester

A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).

General properties

Appearance:	white to off-beige powder
Molecular weight:	588.68
CAS number:	459426-22-3
Molecular formula:	C₂₅H₄₀N₄O₁₀S
Solubility:	water, DMSO, DMF, DCM
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

產品引用

Zhang, L.; Yang, C.; Li, Y.; Niu, S.; Liang, X.; Zhang, Z.; Luo, Q.; Luo, H. Dynamic Changes in the Levels of Amyloid-β 42 Species in the Brain and Periphery of APP/PS1 Mice and Their Significance for Alzheimer’s Disease. Frontiers in Molecular Neuroscience, 2021, 14, 723317. doi: 10.3389/fnmol.2021.723317

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.