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RAPID DiO, lipophilic tracer

貨號 數量 價格 交貨時間
3708-500ug 500 ug –   現貨
3708-5mg 5 mg $250 現貨
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RAPID DiO (also known as FAST DiO™ and Dilinoleyl DiO) is a cyanine dye with green fluorescence, an unsaturated analog of DiO (DiOC18(3)).

RAPID DiO is a lipophilic dye that labels cell membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes. RAPID DiO diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. RAPID DiO has about 50% faster migration kinetics than DiO.

RAPID DiO can be used with other tracers in dual-color studies, such as  RAPID DiI.

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General properties

Appearance: orange plasticine-like
Molecular weight: 923.28
Molecular formula: C54H77F3N2O5S
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 487
ε, L⋅mol−1⋅cm−1: 166000
Emission maximum, nm: 503
Fluorescence quantum yield: 0.1
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