ProteOrange® Protein Quantification Reagent, 500×
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 11210 | 100 uL | – | 現貨 | |
| 41210 | 1 mL | $195 | 現貨 |
ProteOrange® is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.
The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.
The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).
This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.
顧客還購買了這個產品
Hoechst 33342，藍色螢光核酸染色劑 (Hoechst 33342, blue fluorescent nucleic acid stain)
Hoechst 33342（雙苯甲亞胺）是一種細胞可滲透的藍色螢光 雙鏈DNA（dsDNA） 染料，用於對活細胞和固定細胞中的染色體和細胞核進行染色。BDP® FL 馬來醯亞胺 (BDP® FL maleimide)
BDP FL 馬來醯亞胺是一種明亮且光穩定的巰基反應染料，可用於蛋白質標記，是顯微鏡成像用螢光素的理想替代品。THPTA配體
THPTA 是 Cu(I) 催化點擊化學的水溶性配體。該配體使銅穩定在 Cu(I) 氧化態。由於水溶性高，與該配體的反應不需要有機共溶劑。General properties
| Appearance: | Orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
產品引用
- Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
- Grigorenko, V.G.; Krivitskaya, A.V.; Khrenova, M.G.; Rubtsova, M.Y..; Presnova, G.V.; Andreeva, I.P.; Serova, O.V.; Egorov, A.M. Saturation Mutagenesis and Molecular Modeling: The Impact of Methionine 182 Substitutions on the Stability of β-Lactamase TEM-1. International Journal of Molecular Sciences, 2024, 25(14), 7691. doi: 10.3390/ijms25147691
- Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
您的購物車是空的
$ 
