此頁面上的某些內容不提供英文版本,將默認以當前可用的語言顯示。

ProteOrange Protein Quantification Reagent, 500×

貨號 數量 價格 交貨時間
11210 100 uL –   現貨
41210 1 mL $195 現貨
Found better price? Let us know and we will propose the way forward!

ProteOrange is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.

The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.

The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).

This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.

顧客還購買了這個產品

Fluo-4 AM, green fluorescent calcium indicator

Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.

DAPI, blue fluorescent nucleic acid stain

DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent DNA dye used for chromosome staining and as a nuclear counterstain in fluorescence microscopy and flow cytometry.

ProteOrange Protein Gel Stain, 5000×

ProteOrange is a fluorescent dye for the staining of proteins in gels. ProteOrange is an analog of SYPRO orange. It is more sensitive than Coumassie.

General properties

Appearance: Orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 470
Emission maximum, nm: 570

產品引用

  1. Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
  2. Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
已添加您的物品. 查看您的 購物車繼續結賬
物品數量不正確.