ProteOrange® Protein Quantification Reagent, 500×
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
11210 | 100 uL | – | 現貨 | |
41210 | 1 mL | $195 | 現貨 |
ProteOrange® is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.
The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.
The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).
This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.

顧客還購買了這個產品
Annexin V-AF 488 結合物 (Annexin V-AF 488 conjugate)
即用型試劑盒,可使用Annexin V-AF 488 結合物和碘化丙啶標記凋亡和壞死細胞。AF 647 NHS 酯 (AF 647 NHS ester)
明亮的水溶性琥珀醯亞胺酯AF 647染料,用於蛋白質和抗體標記。立即在線獲取免費快遞服務
LUCS® 13, green fluorescent nucleic acid stain
LUCS 13 is a cell-permeant green fluorescent nucleic acid stain that exhibits bright, green fluorescence upon binding to nucleic acids.General properties
Appearance: | Orange solution |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 470 |
Emission maximum, nm: | 570 |
產品引用
- Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
- Grigorenko, V.G.; Krivitskaya, A.V.; Khrenova, M.G.; Rubtsova, M.Y..; Presnova, G.V.; Andreeva, I.P.; Serova, O.V.; Egorov, A.M. Saturation Mutagenesis and Molecular Modeling: The Impact of Methionine 182 Substitutions on the Stability of β-Lactamase TEM-1. International Journal of Molecular Sciences, 2024, 25(14), 7691. doi: 10.3390/ijms25147691
- Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
