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CM-DiI, lipophilic tracer

Neuroscience Cell proliferation Cell membrane Flow cytometry Microscopy

貨號	數量	價格	交貨時間	購買此產品
3513-50ug	50 ug	–	21 天
3513-1mg	1 mg	$349	21 天

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CM-DiI is an orange-red fluorescent carbocyanine dye containing thiol-reactive chloromethyl (CM) moiety. CM-DiI labels plasma membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes.

CM-DiI is used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. The dye is transferred to daughter cells and does not leak to adjacent cells in a population. CM-DiI staining displays bright fluorescence for at least 72 hours (around three to six cell generations). The dye shows little cytotoxicity inside the cell, with minimal effects on the proliferative ability or biology of the cell.

Unlike other membrane dyes, CM-DiI has increased water solubility and can be fixed using traditional aldehyde fixatives. CM-DiI also persists in cells after permeabilization procedures. These make it feasible to detect labeled cells in combination with other histological techniques such as immunochemistry, optical clearing, etc.

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General properties

Appearance:	red powder
Molecular weight:	1142.96
CAS number:	180854-97-1
Molecular formula:	C₆₈H₁₀₅ClIN₃O
Solubility:	in DMSO, DMF, ethanol
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	556
ε, L⋅mol⁻¹⋅cm⁻¹:	129500
Emission maximum, nm:	571
Fluorescence quantum yield:	0.16

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.