LumiTracker® Mito JC-1
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
2275-50ug | 50 ug | – | 現貨 | |
2275-1mg | 1 mg | $110 | 現貨 | |
2275-5mg | 5 mg | $150 | 現貨 |
JC-1 is a cationic carbocyanine dye that accumulates in the mitochondria of live cells in a potential-dependent manner.
The dye exists as a green-fluorescent monomer at depolarized membranes and low concentrations. At higher concentrations (aqueous solutions above 0.1 µM) and hyperpolarized membranes, the dye forms J-aggregates that exhibit an emission at the orange channel. The J-aggregates can be excited at 535 nm, and the monomeric form and aggregate together — at 475 nm.
Healthy cells have high mitochondrial membrane potential, and the decrease of mitochondrial membrane potential is a marker of the early stage of apoptosis. All this allows the use of changes in the orange/green fluorescence ratio of JC-1 to determine healthy vs. depolarized mitochondria. The orange/green fluorescence ratio of JC-1 depends only on the mitochondrial membrane potential and not on other factors such as the size, shape, and density of mitochondria.
顧客還購買了這個產品
Hoechst 33342,藍色螢光核酸染色劑 (Hoechst 33342, blue fluorescent nucleic acid stain)
Hoechst 33342(雙苯甲亞胺)是一種細胞可滲透的藍色螢光 雙鏈DNA(dsDNA) 染料,用於對活細胞和固定細胞中的染色體和細胞核進行染色。DiO,親脂性示蹤劑 (DiO, lipophilic tracer)
用於膜標記和細胞示蹤的綠色螢光親脂染料。DiD,親脂性示蹤劑 (DiD, lipophilic tracer)
用於膜標記和細胞示蹤的遠紅螢光親脂性染料。General properties
Appearance: | red-purple solid |
Molecular weight: | 652.24 |
CAS number: | 47729-63-5; 3520-43-2 |
Molecular formula: | C25H27Cl4IN4 |
IUPAC name: | 1H-Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-, iodide |
Solubility: | good in DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |
產品引用
- Kirsanov, R.S.; Khailova, L.S.; Rokitskaya, T.I.; Lyamzaev, K.G.; Panteleeva, A.A.; Nazarov, P.A.; Firsov, A.M.; Iaubasarova, I.R.; Korshunova, G.A.; Kotova, E.A.; Antonenko, Y.N. Synthesis of Triphenylphosphonium-Linked Derivative of 3,5-Di tert -butyl-4-hydroxybenzylidene-malononitrile (SF6847) via Knoevenagel Reaction Yields an Effective Mitochondria-Targeted Protonophoric Uncoupler. ACS Omega, 2024, 9(10), 11551-11561. doi: 10.1021/acsomega.3c08621