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Biotinyl tyramide

Biotinylation Tyramides Microscopy

貨號	數量	價格	交貨時間	購買此產品
4903-100mg	100 mg	$105	12 天
4903-500mg	500 mg	$439	12 天

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Tyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

This tyramide is a biotin derivative for tyramide amplification and subsequent signal detection with a labeled streptavidin conjugate. It can be used with any antibody or other molecules conjugated to HRP to stain cells and tissues by immunofluorescence methods.

建議協議

Tyramide Signal Amplification (TSA)

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sulfo-Cyanine3 tyramide

A tyramide derivative of sulfo-Cyanine3, an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.

sulfo-Cyanine5.5 tyramide

A tyramide derivative of sulfo-Cyanine5.5, a far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.

sulfo-Cyanine7 tyramide

A tyramide derivative of sulfo-Cyanine7, a near-infrared fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.

General properties

Appearance:	white solid
Molecular weight:	363.48
CAS number:	41994-02-9
Molecular formula:	C₁₈H₂₅N₃O₃S
Solubility:	DMSO, DMF, methanol
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.