LumiTracker® Mito TMRE
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2274-1mg | 1 mg | – | 現貨 | |
| 2274-25mg | 25 mg | $190 | 現貨 |
TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.
In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.
TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).
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DMSO (dimethyl sulfoxide), labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.LumiTracker® Mito JC-1
The cationic carbocyanine dye that is widely used as an indicator of mitochondrial membrane potential to study apoptosis and monitor the health of mitochondria.Ascorbic acid
Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 514.96 |
| CAS number: | 115532-52-0 |
| Molecular formula: | C26H27N2ClO7 |
| IUPAC name: | 3,6-bis(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthylium perchlorate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 156000 |
| Emission maximum, nm: | 575 |
| Fluorescence quantum yield: | 0.66 |
產品引用
- Moiseeva, N.; Eroshenko, D.; Laletina, L.; Rybalkina, E.; Susova, O.; Karamysheva, A.; Tolmacheva, I.; Nazarov, M.; Grishko, V. The Molecular Mechanisms of Oleanane Aldehyde-β-Enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells. Biology, 2023, 12(3), 415. doi: 10.3390/biology12030415
- Kozlova, T.; Popov, A.; Romanov, M. V.; Savintseva, I.; Vasilyeva, D. N.; Baranchikov, A.; Ivanov, V. Ceric Phosphates and Nanocrystalline Ceria: Selective Toxicity to Melanoma Cells. Nanosystems: Physics, Chemistry, Mathematics, 2023, 14, 223–230. doi: 10.17586/2220-8054-2023-14-2-223-230
- Tolmacheva, I.; Beloglazova, Y.; Nazarov, M.; Gagarskikh, O.; Grishko, V. Synthesis and Anticancer Activity of A-Ring-Modified Derivatives of Dihydrobetulin. International Journal of Molecular Sciences, 2023, 24(12), 9863. doi: 10.3390/ijms24129863
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