DusQ 2 CPG 500
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 12960 | 100 mg | – | 現貨 | |
| 32960 | 500 mg |
$530
|
現貨 | |
| 42960 | 1 g |
$990
|
現貨 | |
| 52960 | 5 g | 請查詢 | 現貨 |
This support is intended for automated synthesis of oligonucleotides with 3’-terminal DusQ 2 quencher. Pore size of 500 Å is recommended for the synthesis of oligonucleotides of up to 50 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar® 670, Cyanine5.5, and Quasar® 705.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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Cyanine3疊氮化物 (Cyanine3 azide)
用於 сlick сhemistry 的 Cyanine3 螢光染料的疊氮衍生物。生物素亞磷酰胺（羥基脯氨醇）[Biotin phosphoramidite (hydroxyprolinol)]
生物素亞磷酰胺用於合成生物素化的寡核苷酸。含有二甲氧基三苯甲基基團。
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DusQ 3 CPG 500
DusQ 3 is a dark quencher for far red and near infrared dyes like Cyanine5.5. This is a controlled pore glass solid support for the synthesis of oligonucleotides with the quencher attached to 3'-terminus.
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General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
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