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Alkyne dT phosphoramidite

炔烴 Oligonucleotide synthesis 點擊化學 Phosphoramidites

貨號	數量	價格	交貨時間
1897-100mg	100 mg	$195	現貨
1897-1g	1 g	$1250	現貨
1897-5g	5 g	$2850	現貨

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This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to the deoxyuridine fragment, and it does not interfere with the hybridization of the complementary strand. This allows the preparation of modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain by virtue of copper-catalyzed Click chemistry reaction with various azides.

This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.

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Cyanine5.5 NHS酯 (Cyanine5.5 NHS ester)

Cyanine5.5 NHS 是一種遠紅/近紅外胺反應性染料。

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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.

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DusQ® 1 phosphoramidite

A black quencher dye phosphoramidite for the synthesis of oligonucleotide probes labeled with DusQ® 1 quenchers at 5′-terminal. Useful for HEX, JOE, ROX, Cyanine3.

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General properties

Appearance:	off white solid
Mass spec M+ increment:	437.1
Molecular weight:	877.96
Molecular formula:	C₄₈H₅₆N₅O₉P
Solubility:	good in acetonitrile, DCM
Quality control:	NMR ¹H, NMR ³¹P, HPLC-MS
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.