Alkyne dT phosphoramidite
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
1897-100mg | 100 mg | $330 $195 | 現貨 | |
1897-1g | 1 g | $2590 $1250 | 現貨 | |
1897-5g | 5 g | $9900 $2850 | 現貨 |
This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to the deoxyuridine fragment, and it does not interfere with the hybridization of the complementary strand. This allows the preparation of modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain by virtue of copper-catalyzed Click chemistry reaction with various azides.
This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.
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General properties
Appearance: | off white solid |
Mass spec M+ increment: | 437.1 |
Molecular weight: | 877.96 |
Molecular formula: | C48H56N5O9P |
Solubility: | good in acetonitrile, DCM |
Quality control: | NMR 1H, NMR 31P, HPLC-MS |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |