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Alkyne dT phosphoramidite

貨號 數量 價格 交貨時間
1897-100mg 100 mg $330 $195 現貨
1897-1g 1 g $2590 $1250 現貨
1897-5g 5 g $9900 $2850 現貨
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This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to the deoxyuridine fragment, and it does not interfere with the hybridization of the complementary strand. This allows the preparation of modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain by virtue of copper-catalyzed Click chemistry reaction with various azides.

This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.

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General properties

Appearance: off white solid
Mass spec M+ increment: 437.1
Molecular weight: 877.96
Molecular formula: C48H56N5O9P
Solubility: good in acetonitrile, DCM
Quality control: NMR 1H, NMR 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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