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VIC phosphoramidite, 6-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| BH260 | 50 mg | – | 現貨 | |
| DH260 | 250 mg |
$490
|
現貨 | |
| FH260 | 1 g |
$990
|
現貨 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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ATT 565 疊氮化物 (ATT 565 azide)
水溶性橙色螢光染料 ATT 565 的疊氮衍生物，用於標記含炔基的生物分子和生物體的螢光視覺化。純6-異構體。磺基-Cyanine5疊氮化物 (sulfo-Cyanine5 azide)
水溶性磺基-Cyanine5 疊氮化物螢光染料，用於點擊化學的。JOE 疊氮化物，5-異構體 (JOE azide, 5-isomer)
JOE 是一種氯代螢光素染料，常用於寡核苷酸的 HEX 通道。它的疏水性比 HEX 弱。是一種疊氮衍生物，適用於點擊化學。General properties
| Appearance: | white / off white solid |
| Molecular weight: | 1023.39 |
| CAS number: | 1414265-81-8 |
| Molecular formula: | C52H59Cl3N3O10P |
| Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 525 |
| ε, L⋅mol−1⋅cm−1: | 103000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.53 |
| CF260: | 0.07 |
| CF280: | 0.07 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | standard deprotection conditions |
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