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VIC phosphoramidite, 6-isomer
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
BH260 | 50 mg | – | 現貨 | |
DH260 | 250 mg | $490 | 現貨 | |
FH260 | 1 g | $990 | 現貨 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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FAM-dT亞磷醯胺
亞磷醯胺用於在合成序列中間,用螢光素 (FAM) 標記寡核苷酸。
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DusQ 2 phosphoramidite
DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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ROX 疊氮化物,6-異構體 (ROX azide, 6-isomer)
ROX(羅丹明 X、羅丹明 101)染料的疊氮化物衍生物,用於通過點擊化學進行生物分子標記。純6-異構體。General properties
Appearance: | white / off white solid |
Molecular weight: | 1023.39 |
CAS number: | 1414265-81-8 |
Molecular formula: | C52H59Cl3N3O10P |
Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | standard deprotection conditions |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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