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VIC phosphoramidite, 6-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| BH260 | 50 mg | – | 現貨 | |
| DH260 | 250 mg |
$490
|
現貨 | |
| FH260 | 1 g |
$990
|
現貨 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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R6G NHS ester, 5-isomer
R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.Cyanine5.5 amine
Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.
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DusQ 21 CPG 500
DusQ 21 is a quencher for dyes in red part of the spectrum, including Cyanine5 and Cyanine5.5.
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General properties
| Appearance: | white / off white solid |
| Molecular weight: | 1023.39 |
| CAS number: | 1414265-81-8 |
| Molecular formula: | C52H59Cl3N3O10P |
| Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 525 |
| ε, L⋅mol−1⋅cm−1: | 103000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.53 |
| CF260: | 0.07 |
| CF280: | 0.07 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | standard deprotection conditions |
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