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R6G NHS ester, 5-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 14220 | 1 mg | $110 | 現貨 | |
| 24220 | 5 mg | $210 | 現貨 | |
| 44220 | 25 mg |
$410
|
現貨 | |
| 54220 | 50 mg |
$695
|
現貨 | |
| 64220 | 100 mg |
$1190
|
現貨 |
Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.
This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.
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一種高靈敏度染色劑，用於電泳凝膠中的 dsDNA、ssDNA 和 RNA 染色。General properties
| Appearance: | dark colored solid |
| Molecular weight: | 555.58 |
| Molecular formula: | C31H29N3O7 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
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