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Universal CPG type II, 500A

Oligonucleotide synthesis CPG modifier solid supports
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3219-1g 1 g $45 現貨
3219-10g 10 g $410 現貨
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The Universal CPG type II, 500A is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.

The Universal CPG type II, 500A is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 120 bases. For oligos up to 120 bases universal support 1000 Å can be used.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 2 hours at 80°C or 8 hours at 55°C using concentrated ammonia; 15 minutes at 65°C using AMA mixture, ammonium hydroxide - 40% methylamine (1:1).

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通用 CPG II 型，1000A (Universal CPG type II, 1000A)

這種通用支持物旨在用於寡核苷酸的自動合成，以加速解封閉過程中的去磷酸化過程。對於多達 120 個堿基的寡核苷酸合成，建議使用 1000 Å 的孔徑。

General properties

Appearance: white powder
Quality control: loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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產品規格

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonium hydroxide 2 hours at 80 °C or AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 15 minutes at 65 °C
Deprotection conditions: identical to protected nucleobases
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