Universal CPG type II, 500A
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
3219-1g | 1 g |
$45
|
現貨 | |
3219-10g | 10 g |
$410
|
現貨 | |
3219-100g | 100 g |
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21 天 |
The Universal CPG type II, 500 Å is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.
The Universal CPG type II, 500 Å is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases. For oligos up to 120 bases universal support 1000 Å or 1400 Å can be used.
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General properties
Appearance: | white powder |
Quality control: | loading measurement, functional testing in oligo synthesis. |
Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |
Oligo synthesis details
Pore size, Å: | 500 |
Typical loading, umol/g: | 50−80 |
Coupling conditions: | standard conditions for universal CPG |
Cleavage conditions: | 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide- 40% methylamine (1:1) |
Deprotection conditions: | identical to protected nucleobases |
