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Universal CPG type I, 500A
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我們建議使用Universal CPG type II, 500A作為替代品
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2646-1g | 1 g | 請查詢 | 現貨 (1 pcs) |
The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.
The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1) for a 50 nmol scale synthesis. The bigger scale of synthesis requires more than 4 hours to complete.
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DusQ 1 CPG 1000
Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
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DusQ 2 CPG 1000
Controlled pore glass (CPG) 1000 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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DusQ 1 amidite, 5'-terminal
Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ 1 quencher.
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General properties
| Appearance: | white to cream beads |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C |
| Deprotection conditions: | identical to protected nucleobases |
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