Thiazole Orange NHS ester
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
2183-1mg | 1 mg |
–
|
現貨 | |
2183-5mg | 5 mg |
$60
|
現貨 |
Thiazol Orange (TO) is an asymmetric cyanine dye whose fluorescence highly depends on the local environment. TO is essentially dark in solution; however, its fluorescence increases a thousandfold when ТО is introduced into double-stranded DNA and RNA (dsDNA or dsRNA). When TO-labeled oligonucleotide is hybridized to its complementary sequence, TO acts as an intercalator.
TO fluorescence is also induced by interaction with supramolecular hosts and amino acid side chains, which allows it to be used for detecting not only nucleic acids and proteins but also other analytes such as ions and small molecules.
The maximum absorption of TO with DNA is 509 nm, and the maximum emission is 532 nm.
Thiazole Orange NHS ester effectively reacts with amines and is well suited for labeling biomolecules (peptides, proteins, amino-DNA, etc.) in an aqueous medium. Thiazole Orange NHS ester can be useful for studying the binding of nucleic acids to other biomolecules, such as DNA-binding proteins.

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螢光素的氨基衍生物,純5-異構體。在脂肪胺基團和螢光團之間包含一個連接臂。可用於酶促轉氨基反應的標記、與親電試劑的反應以及胺化還原反應。General properties
Appearance: | orange powder |
Molecular weight: | 538.07 |
Molecular formula: | C28H28ClN3O4S |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 509 |
Emission maximum, nm: | 532 |
