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AF 647 DBCO

Cycloalkynes AF 647 Copper-free click chemistry
貨號 數量 價格 交貨時間
1G8F0 1 mg $140 現貨
2G8F0 5 mg $450 現貨
4G8F0 25 mg $1460 現貨
5G8F0 50 mg $1890 現貨
6G8F0 100 mg $2990 現貨
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.

AF 647 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

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AF 568 DBCO

Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.

AF 488 DBCO

含有綠色熒光團 AF 488的二苯並環辛炔（DBCO、ADIBO），可用於標記活細胞、整個生物體和無生命樣品內的疊氮化物生物分子。純5-異構體。
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AF 647 NHS 酯 (AF 647 NHS ester)

明亮的水溶性琥珀醯亞胺酯AF 647染料，用於蛋白質和抗體標記。

General properties

Appearance: deep-blue powder
Molecular weight: 1266.74
Molecular formula: C56H68K3N4O14S4
Solubility: water, DMSO, DMF, methanol
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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