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ROX maleimide, 5-isomer

ROX 馬來酰亞胺
貨號 數量 價格 交貨時間
11280 1 mg $110 5 天
21280 5 mg $165 現貨
41280 25 mg $285 現貨
51280 50 mg $495 現貨
61280 100 mg $880 現貨
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ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.

This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.

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General properties

Appearance: red powder
Mass spec M+ increment: 656.3
Molecular weight: 656.73
Molecular formula: C39H36N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00
CF260: 0.62
CF280: 0.49

產品引用

  1. Khan, S.; Molloy, J.E.; Puhl, H.; Schulman, H.; Vogel, S.S. Real-time single-molecule imaging of CaMKII-calmodulin interactions. Biophysical Journal, 2024, 123(7), 824-838. doi: 10.1016/j.bpj.2024.02.021
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