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sulfo-Cyanine3.5 alkyne

sulfo-Cyanine3.5 Alkynes Click chemistry
貨號 數量 價格 交貨時間
A23B0 1 mg $125 現貨
B23B0 5 mg $260 現貨
C23B0 10 mg $325 現貨
D23B0 25 mg $510 現貨
E23B0 50 mg $895 現貨
F23B0 100 mg $1490 現貨
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sulfo-Cyanine3.5 is a dye with fluorescence in the orange spectrum range (absorption maximum at 576 nm; emission maximum at 603 nm). Its absorption and emission spectra are between that of Cyanine3 and Cyanine5. Therefore, sulfo-Cyanine3.5 is commonly used as a FRET-donor in a pair with Cyanine5, as a FRET-acceptor in a pair with Cyanine3, and in BRET experiments.

sulfo-Cyanine3.5 is a sulfonated derivative with four sulfo groups, which allows its high hydrophilicity and performing all reactions with this reagent in aqueous solutions without adding organic solvents.

The reagent is an alkyne derivative of sulfo-Cyanine3.5 dye for copper-catalyzed click chemistry. The terminal alkynyl (acetylene) group allows conjugating the fluorophore with the molecules containing an azide group through 1,3-dipolar cycloaddition in the presence of a copper(I) catalyst.

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3-Azidopropanol

Azidopropanol is a short bifunctional linker compound with azide and hydroxyl functional groups.
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sulfo-Cyanine3.5 NHS ester

Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.

AF 488 alkyne

Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides. Pure 5-isomer.

General properties

Appearance: dark colored solid
Mass spec M+ increment: 914.2
Molecular weight: 1028.32
Molecular formula: C41H40N3K3O13S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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Spectral properties

Excitation/absorption maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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