HMRhoNox-M, Fe(II)-selective fluorescent probe
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3317-50ug | 50 ug | $120 | 現貨 | |
| 3317-500ug | 500 ug |
$590
|
現貨 |
HMRhoNox-M (also known as LysoRhoNox) is a Fe2+-selective fluorescent probe based on the N-oxide-controlled spirocyclization of tetramethyl-hydroxymethyl rhodamine.
In the absence of Fe2+, HMRhoNox-M exists in the non-fluorescent spirocyclic form showing only negligible fluorescence in an aqueous buffer and at physiological pH. The addition of Fe2+ induces a 60-fold increase of the fluorescence signal at 575 nm through the deoxygenation of the dialkylamino group and the transition of the probe to an open fluorescent form. HMRhoNox-M responds to Fe2+ in a dose-dependent manner.
The fluorescence response of HMRhoNox-M is highly selective for Fe2+ over other transition metal ions, including Fe3+, alkali metal ions, and alkaline earth metal ions.
HMRhoNox-M is the cell-permeant probe that is mainly localized in lysosomes. It is suitable for monitoring fluctuations of endogenous labile iron in living cells, including the transferrin-induced Fe uptake.
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| Appearance: | beige-pinkish crystals |
| Molecular weight: | 388.47 |
| Molecular formula: | C24H24N2O3 |
| Solubility: | DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| Emission maximum, nm: | 575 |
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