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C6 Spacer phosphoramidite

Phosphoramidites

貨號	數量	價格	交貨時間
24160	250 mg	$269 $169	現貨
44160	1 g	$837 $642	現貨
74160	10 g	請查詢	21 天

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C6 spacer phosphoramidite serves as a crucial tool in nucleic acid chemistry, enabling researchers to design more effective oligonucleotides by incorporating hydrophobic spacers that enhance performance in various biochemical applications. Its ability to minimize unwanted interactions while providing structural flexibility makes it an invaluable reagent in modern molecular biology techniques. Multiple spacers can be added to achieve precise control over the length of the spacer arms, which is essential in studies involving hairpin loops and duplex formations.

The C6 spacer is hydrophobic, making it suitable for applications where water solubility is not critical. It effectively distances fluorescent dyes or other modifications from the oligonucleotide sequence, reducing quenching effects. For example, G-rich sequences can quench fluorescein; adding a spacer helps mitigate this issue.

This spacer can be utilized in various contexts, including PCR amplification and hybridization assays. It allows for flexibility in the design of probes and primers, which can be crucial for specific applications like Scorpion Primers and SMART detection assays.

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FAM 疊氮化物，6-異構體 (FAM azide, 6-isomer)

FAM（螢光素）疊氮化物，6-異構體，是一種常用於點擊化學的螢光染料疊氮化物。

C8-Alkyne-dT-CE-phosphoramidite

C8-Alkyne-dT-CE-phosphoramidite is a specialized reagent used in the synthesis of oligonucleotides that incorporates a single dT alkyne group. This compound is particularly valuable for applications involving click chemistry, allowing for efficient conjugation reactions.

Alkyne dT phosphoramidite

Phosphoramidite modifier for synthesizing oligonucleotides containing alkyne modification in the middle of the chain.

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General properties

Appearance:	colorless oil
Molecular weight:	620.77
Molecular formula:	C₃₆H₄₉N₂O₅P
Solubility:	DCM, acetonitrile, DMF, DMSO. Sensitive to moisture in solvents
Quality control:	NMR ¹H, ³¹P, HPLC-MS (95%), functional testing
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

Oligo synthesis details

Diluent:	anhydrous acetonitrile
Coupling conditions:	standard conditions identical to normal nucleobases. Tolerates oxidation step conditions.
Cleavage conditions:	in concentrated ammonium hydroxide for 5 hours at 60 °C (or 1 hour for fast-deprotecting amidites). AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.

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