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Alkyne dT phosphoramidite

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This product will be discontinued soon in favor of Alkyne dT phosphoramidite. We may still have some material in stock (please see availability above).

Alkyne dT phosphoramidite is used for the synthesis of oligonucleotides containing an internal alkyne modification attached to the uridine 5-position. The alkyne group can be subsequently modified by various azides using copper-catalyzed Click chemistry.

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DusQ 1 phosphoramidite

A black quencher dye phosphoramidite for the synthesis of oligonucleotide probes labeled with DusQ 1 quenchers at 5’-terminal. Useful for HEX, JOE, ROX, Cyanine3.
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DusQ 2 phosphoramidite

DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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R6G 疊氮化物,5-異構體 (R6G azide, 5-isomer)

羅丹明 6G 是一種氧雜蒽染料,已用於 DNA 測序和 qPCR。這種疊氮化物衍生物用於通過點擊化學將染料附著到 DNA 和其他分子上。

General properties

Appearance: white solid
Molecular weight: 935.01
Molecular formula: C50H59N6O10P
Solubility: good in THF, DCM, acetonitrile
Quality control: NMR 1H, HPLC-MS (90+%), functional testing in oligo synthesis
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Oligo synthesis details

Coupling conditions: 6 min. coupling time; 45 sec. oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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