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AF 647 maleimide

貨號 數量 價格 交貨時間
16880 1 mg $140 現貨
26880 5 mg $450 現貨
46880 25 mg $1460 現貨
56880 50 mg $1890 現貨
66880 100 mg $2990 現貨
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AF 647 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 647 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.

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DMSO (dimethyl sulfoxide), labeling grade

Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.

Biotin-PEG4-NHS ester

PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.

General properties

Appearance: dark blue powder
Molecular weight: 1081.40
Molecular formula: C41H47K3N4O15S4
Solubility: good in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (90+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08
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