AF 488 TFP ester
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 118T0 | 1 mg | $165 | 現貨 | |
| 218T0 | 5 mg |
$510
|
現貨 | |
| 418T0 | 25 mg |
$1650
|
現貨 |
AF 488 is a bright and photostable dye well suited for labeling sensitive proteins and antibodies due to its hydrophilicity. AF 488 is a sulfonated derivative of Rhodamine 110 (R110), and, like other rhodamines, AF 488 is represented by two (5- and 6-) isomers. Both isomers have almost identical photophysical properties but require their separation since using a mixture of isomers leads to the «doubling» of the peaks of the labeled products during HPLC and electrophoretic separation. This product contains isomerically pure 5-AF 488.
Tetrafluorophenyl (TFP) esters belong to a group of activated esters widely used to conjugate fluorophores to the primary and secondary amines of biomolecules. Like hydroxysuccinimide (NHS) esters, they form a strong amide bond between the dye and the compound being modified; however, TFP esters are more stable at the basic pH values typically associated with reactions with amines and are also less susceptible to spontaneous hydrolysis.
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AF 532 NHS ester
NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.AF 594 NHS酯 (AF 594 NHS ester)
AF 594 染料的紅色螢光 NHS-酯，用於標記蛋白質、肽、抗體和任何含有 -NH2 基團的分子。純5-異構體
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General properties
| Appearance: | red crystals |
| Molecular weight: | 681.53 |
| CAS number: | 2133404-55-2 |
| Molecular formula: | C27H13F4N2O11S2- |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
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