5-Ethynyl-uridine (EU)
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2439-25mg | 25 mg | $137 | 現貨 | |
| 2439-100mg | 100 mg |
$487
|
現貨 | |
| 2439-500mg | 500 mg |
$950
|
現貨 |
5-Ethynyl-uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.
EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyluridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.
EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via click chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.
5-Ethynyl-uridine has an advantage over its analog, 5-bromo-uridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromo-uridine. Thus, the click chemistry approach allows whole-mount staining of large samples like organs or tissue fragments.
顧客還購買了這個產品
Biotin-PEG2-alkyne
Biotin alkyne is a reagent for click chemistry labeling with biotin, an affinity probe to streptavidin.TAMRA DBCO, 5-isomer
Cyclooctyne derivative of tetramethylrhodamine (TMR, TAMRA) for copper-free click chemistry conjugation with azides. Pure 5-isomer.HPG (L-Homopropargylglycine)
Noncanonical amino acid analog of methionine with a terminal alkyne moiety for nascent protein synthesis detection.General properties
| Appearance: | off white solid |
| Molecular weight: | 268.22 |
| CAS number: | 69075-42-9 |
| Molecular formula: | C11H12N2O6 |
| IUPAC name: | 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | 下載 |
| 產品規格 |
您的購物車是空的
$ 
