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FAM phosphoramidite, 5-isomer

Oligonucleotide synthesis FAM（螢光素） Phosphoramidites

貨號	數量	價格	交貨時間
2639-500mg	500 mg	–	現貨
2639-1g	1 g	$190 $147	現貨
2639-5g	5 g	$740 $629	現貨
2639-10g	10 g	$1290 $970	現貨
2639-50g	50 g	請查詢	21 天
2639-100g	100 g	請查詢	21 天

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Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

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Cyanine5.5亞磷醯胺 (Cyanine5.5 phosphoramidite)

Amidite 用於合成5' 末端帶有Cyanine5.5染料的寡核苷酸和 qPCR 探針。適用於多重 qPCR。

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FAM 亞磷酰胺，6-異構體 (FAM phosphoramidite, 6-isomer)

單一構體 6-FAM 亞磷酰胺，用於寡核苷酸的修飾。

JOE 疊氮化物，5-異構體 (JOE azide, 5-isomer)

JOE 是一種氯代螢光素染料，常用於寡核苷酸的 HEX 通道。它的疏水性比 HEX 弱。是一種疊氮衍生物，適用於點擊化學。

General properties

Appearance:	off white solid
Molecular weight:	843.94
CAS number:	147566-42-5
Molecular formula:	C₄₆H₅₈N₃O₁₀P
Solubility:	Good solubility in acetonitrile and DCM
Quality control:	NMR ¹H and ³¹P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	492
ε, L⋅mol⁻¹⋅cm⁻¹:	72000
Emission maximum, nm:	517
Fluorescence quantum yield:	0.93
CF₂₆₀:	0.22
CF₂₈₀:	0.17

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	coupling time 10 min
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

產品引用

Zharkov, T. D.; Markov, O. V.; Zhukov, S. A.; Khodyreva, S. N.; Kupryushkin, M. S. Influence of Combinations of Lipophilic and Phosphate Backbone Modifications on Cellular Uptake of Modified Oligonucleotides. Molecules, 2024, 29(2), 452. doi: 10.3390/molecules29020452
Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.