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VIC phosphoramidite, 6-isomer
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
BH260 | 50 mg | – | 現貨 | |
DH260 | 250 mg | $490 | 現貨 | |
FH260 | 1 g | $990 | 現貨 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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Cyanine5疊氮化物
Cyanine5 疊氮化物是一種用 Cyanine5(一種流行的螢光染料)進行點擊化學標記的試劑。
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Alkyne dT phosphoramidite
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
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dsGreen 用於實時定量PCR, 100× (dsGreen for Real-Time PCR, 100×)
用於實時定量PCR 的 dsGreen 儲備液,100×General properties
Appearance: | white / off white solid |
Molecular weight: | 1023.39 |
CAS number: | 1414265-81-8 |
Molecular formula: | C52H59Cl3N3O10P |
Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | standard deprotection conditions |
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