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Universal CPG type I, 500A

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The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.

The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1) for a 50 nmol scale synthesis. The bigger scale of synthesis requires more than 4 hours to complete.

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DusQ® 2 CPG 500

Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ® 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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DusQ® 21 CPG 500

DusQ® 21 is a quencher for dyes in red part of the spectrum, including Cyanine5 and Cyanine5.5.
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DusQ 1 CPG 1000

Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
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General properties

Appearance: white to cream beads
Quality control: loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C
Deprotection conditions: identical to protected nucleobases
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