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TR hydrazide, 5-isomer

醯肼類 TR

貨號	數量	價格	交貨時間
2Y270	5 mg	$169	7 天
4Y270	25 mg	$590	12 天
6Y270	100 mg	$1490	12 天

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This product is a hydrazide derivative of TR dye. Hydrazides efficiently react with aldehydes and ketones, resulting in hydrazones, so that this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with hydrazides). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can also be conjugated with hydrazides in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

TR is a red-fluorescent dye used for cell staining, fluorescence microscopy applications, and cell sorting with fluorescent-activated cell sorting machines. TR is also commonly used in molecular biology, mainly quantitative RT-PCR and cellular assays.

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General properties

Appearance:	dark fine crystals
Molecular weight:	620.75
Molecular formula:	C₃₁H₃₂N₄O₆S₂
Solubility:	good in methanol, DMSO, DCM; moderate in acetonitrile; poor soluble in water
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	582
ε, L⋅mol⁻¹⋅cm⁻¹:	98000
Emission maximum, nm:	600
Fluorescence quantum yield:	0.79

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.