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AF 430 hydrazide

AF 430 Hydrazides

貨號	數量	價格	交貨時間
10870	1 mg	$110	現貨
20870	5 mg	$210	現貨
40870	25 mg	$410	現貨
50870	50 mg	$695	現貨
60870	100 mg	$1190	現貨

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AF 430 is a fluorescent dye with an excitation maximum at 430 nm and an emission maximum at 542 nm. AF 430 is one of the few dyes that absorb between 400 nm and 450 nm. AF 430 fluorescence is photostable and pH-insensitive in a broad range of pH values.

This product is a hydrazide derivative of AF 430 dye. Hydrazides efficiently react with aldehydes and ketones, resulting in hydrazones, so that this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with hydrazides). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can also be conjugated with hydrazides in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

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AF 430 maleimide

AF 430 is a green-yellow emitting fluorescent dye of the coumarin series. This maleimide derivative allows the labeling of thiolated biomolecules.

ssGreen RNA Gel Staining Solution, 10,000×

A highly sensitive stain for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels.

AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.

General properties

Appearance:	yellow powder
Mass spec M+ increment:	499.1
Molecular weight:	517.52
Molecular formula:	C₂₂H₂₆N₃F₃O₆S
Solubility:	good in DMF, DMSO, water
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	430
ε, L⋅mol⁻¹⋅cm⁻¹:	15955
Emission maximum, nm:	542
Fluorescence quantum yield:	0.23

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.

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