LumiTracker® Mito TMRE
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
2274-1mg | 1 mg | – | 現貨 | |
2274-25mg | 25 mg | $190 | 現貨 |
TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.
In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.
TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).
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BDP® 493/503 lipid stain
BDP 493/503 is a lipophilic green fluorescent dye for the staining and tracing of cell membranes, lipids, and oils.Cyanine3 NHS酯
胺反應性 Cyanine3 染料 NHS 酯。LUCS® 13, green fluorescent nucleic acid stain
LUCS 13 is a cell-permeant green fluorescent nucleic acid stain that exhibits bright, green fluorescence upon binding to nucleic acids.General properties
Appearance: | dark colored solid |
Molecular weight: | 514.96 |
CAS number: | 115532-52-0 |
Molecular formula: | C26H27N2ClO7 |
IUPAC name: | 3,6-bis(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthylium perchlorate |
Solubility: | good in DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 552 |
ε, L⋅mol−1⋅cm−1: | 156000 |
Emission maximum, nm: | 575 |
Fluorescence quantum yield: | 0.66 |
產品引用
- Moiseeva, N.; Eroshenko, D.; Laletina, L.; Rybalkina, E.; Susova, O.; Karamysheva, A.; Tolmacheva, I.; Nazarov, M.; Grishko, V. The Molecular Mechanisms of Oleanane Aldehyde-β-Enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells. Biology, 2023, 12(3), 415. doi: 10.3390/biology12030415
- Kozlova, T.; Popov, A.; Romanov, M. V.; Savintseva, I.; Vasilyeva, D. N.; Baranchikov, A.; Ivanov, V. Ceric Phosphates and Nanocrystalline Ceria: Selective Toxicity to Melanoma Cells. Nanosystems: Physics, Chemistry, Mathematics, 2023, 14, 223–230. doi: 10.17586/2220-8054-2023-14-2-223-230
- Tolmacheva, I.; Beloglazova, Y.; Nazarov, M.; Gagarskikh, O.; Grishko, V. Synthesis and Anticancer Activity of A-Ring-Modified Derivatives of Dihydrobetulin. International Journal of Molecular Sciences, 2023, 24(12), 9863. doi: 10.3390/ijms24129863