TAMRA phosphoramidite, 5-isomer
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2497-100mg | 100 mg | 請查詢 | 現貨 (3 pcs) | |
| 2497-250mg | 250 mg | 請查詢 | 現貨 (2 pcs) | |
| 2497-1g | 1 g | 請查詢 | 現貨 (2 pcs) | |
| 2497-5g | 5 g | 請查詢 | 現貨 (2 pcs) |
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
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BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.THPTA配體
THPTA 是 Cu(I) 催化點擊化學的水溶性配體。該配體使銅穩定在 Cu(I) 氧化態。由於水溶性高，與該配體的反應不需要有機共溶劑。DusQ 1 CPG 500
固體支持物-可控微孔玻璃 (CPG) 500，用於合成3' 末端帶有 DusQ 1 猝滅劑的寡核苷酸。與標準解封閉條件兼容。
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General properties
| Appearance: | purple solid |
| Mass spec M+ increment: | 589.60 |
| Molecular weight: | 727.83 |
| Molecular formula: | C40H50N5O6P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, HPLC-MS (95%), coupling test |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Diluent: | acetonitrile |
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