sulfo-Cyanine5.5 tyramide
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3858-1mg | 1 mg | $110 | 現貨 |
Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble far-red fluorescent dye sulfo-Cyanine5.5. sulfo-Cyanine5.5 tyramide (also known as Cy5.5® and Cyanine5.5 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.
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BDP TMR X NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.sulfo-Cyanine7 tyramide
A tyramide derivative of sulfo-Cyanine7, a near-infrared fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.BDP TR X NHS 酯 (BDP TR X NHS ester)
BDP TR X NHS 酯是 BDP TR 的衍生物，含有基於氨基己酸 (C6) 的長連接體。該染料的吸收和發射接近ROX染料。NHS 酯可用於偶合蛋白質和肽。General properties
| Appearance: | dark blue solid |
| Molecular weight: | 1136.48 |
| Molecular formula: | C48H48K3N3O14S4 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 673 |
| ε, L⋅mol−1⋅cm−1: | 211000 |
| Emission maximum, nm: | 691 |
| Fluorescence quantum yield: | 0.21 |
| CF260: | 0.09 |
| CF280: | 0.11 |
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